Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells

Acta Pharmacol Sin. 2005 Feb;26(2):223-7. doi: 10.1111/j.1745-7254.2005.00035.x.


Aim: To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F (TWHF), on the co-stimulatory molecule expression and interleukin-12 (IL-12) production from THP-1 cells.

Methods: THP-1 cells were differentiated into macrophage-like cells by Me2SO, and then cultured with IFN-gamma (500 kU/L) and lipopolysaccharide (LPS) (1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on a FACScan flow cytometer. IL-12p40, IL-12p70 were assayed by ELISA.

Results: Triptolide suppressed CD80 and CD86 expressions on IFN-gamma (500 kU/L) and LPS (1 mg/L) activated THP-1 cells at nontoxic dosages of 2.5-0.625 microg/L. Furthermore, the production of IL-12p40 and IL-12p70 were also significantly reduced in THP-1 cells exposed to triptolide.

Conclusion: Triptolide impairs the antigen-presenting function by inhibiting CD80 and CD86 expressions and decreased IL-12p40 and IL-12p70 (bioactive form) productions from the activated THP-1 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism*
  • B7-1 Antigen / metabolism*
  • B7-2 Antigen
  • Cell Line, Tumor
  • Diterpenes / isolation & purification
  • Diterpenes / pharmacology*
  • Epoxy Compounds
  • Humans
  • Immunosuppressive Agents / pharmacology
  • Interleukin-12 / biosynthesis*
  • Interleukin-12 Subunit p40
  • Leukemia, Myeloid / pathology
  • Membrane Glycoproteins / metabolism*
  • Monocytes / metabolism
  • Phenanthrenes / isolation & purification
  • Phenanthrenes / pharmacology*
  • Plants, Medicinal / chemistry
  • Protein Subunits / biosynthesis
  • Tripterygium* / chemistry


  • Antigens, CD
  • B7-1 Antigen
  • B7-2 Antigen
  • CD86 protein, human
  • Diterpenes
  • Epoxy Compounds
  • Immunosuppressive Agents
  • Interleukin-12 Subunit p40
  • Membrane Glycoproteins
  • Phenanthrenes
  • Protein Subunits
  • Interleukin-12
  • triptolide