Natural HIV-1 NEF Accelerates Virus Replication in Primary Human Lymphocytes

Virology. 1992 May;188(1):391-5. doi: 10.1016/0042-6822(92)90772-h.

Abstract

HIV-1 NEF genes were isolated directly from peripheral blood lymphocyte DNA of two HIV-1-infected individuals and cloned into an HXB-2-infectious molecular clone. The effect of NEF on virus production in T-cell lines and primary human lymphocytes was studied. Naturally occurring NEF accelerates virus production in primary human lymphocytes, but not in T-cell lines.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Viral
  • Gene Products, nef / chemistry
  • Gene Products, nef / genetics
  • Genes, nef*
  • HIV Long Terminal Repeat
  • HIV-1 / genetics*
  • HIV-1 / physiology
  • Humans
  • Kinetics
  • Lymphocytes / microbiology
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Alignment
  • T-Lymphocytes / microbiology
  • Virus Replication / genetics
  • nef Gene Products, Human Immunodeficiency Virus

Substances

  • DNA, Viral
  • Gene Products, nef
  • nef Gene Products, Human Immunodeficiency Virus

Associated data

  • GENBANK/M84627
  • GENBANK/M84628
  • GENBANK/M84629
  • GENBANK/M84630
  • GENBANK/M84631
  • GENBANK/M84632