Abstract
Functional supramolecular chemistry at the single-molecule level. Single strands of DNA can be captured inside α-hemolysin transmembrane pore protein to form single-species α-HL·DNA pseudorotaxanes. This process can be used to identify a single adenine nucleotide at a specific location on a strand of DNA by the characteristic reductions in the α-HL ion conductance. This study suggests that α-HL-mediated single-molecule DNA sequencing might be fundamentally feasible.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacterial Toxins / genetics
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Base Sequence
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DNA / chemistry*
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DNA, Single-Stranded / chemistry
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Deoxyribonucleotides / analysis*
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Electrochemistry
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Hemolysin Proteins
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Ion Transport
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Poly A / chemistry
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Poly C / chemistry
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Porins / genetics
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Rotaxanes / chemistry
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Sequence Analysis, DNA / methods*
Substances
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Bacterial Toxins
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DNA, Single-Stranded
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Deoxyribonucleotides
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Hemolysin Proteins
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Porins
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Rotaxanes
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staphylococcal alpha-toxin
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Poly A
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poly(dA)
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poly(dC)
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Poly C
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DNA