Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method

Tianyan Song; Claudia Toma; Noboru Nakasone; Masaaki Iwanaga

doi:10.1016/j.femsle.2004.12.014

Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method

FEMS Microbiol Lett. 2005 Feb 1;243(1):259-63. doi: 10.1016/j.femsle.2004.12.014.

Authors

Tianyan Song¹, Claudia Toma, Noboru Nakasone, Masaaki Iwanaga

Affiliation

¹ Division of Bacterial Pathogenesis, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan. k018763@med.u-ryukyu.ac.jp

PMID: 15668027
DOI: 10.1016/j.femsle.2004.12.014

Abstract

Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Antigens, Bacterial / genetics
Bacterial Proteins / genetics
DNA Probes
Diarrhea / microbiology
Dysentery, Bacillary / microbiology
Escherichia coli / genetics
Escherichia coli / isolation & purification*
Escherichia coli Infections / microbiology
Feces / microbiology
Humans
Nucleic Acid Amplification Techniques / methods*
Polymerase Chain Reaction / methods
Sensitivity and Specificity
Shigella / genetics
Shigella / isolation & purification*
Time Factors

Substances

Antigens, Bacterial
Bacterial Proteins
DNA Probes
ipaH protein, Shigella flexneri