Sensitive and Rapid Detection of Shigella and Enteroinvasive Escherichia Coli by a Loop-Mediated Isothermal Amplification Method

FEMS Microbiol Lett. 2005 Feb 1;243(1):259-63. doi: 10.1016/j.femsle.2004.12.014.

Abstract

Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Antigens, Bacterial / genetics
  • Bacterial Proteins / genetics
  • DNA Probes
  • Diarrhea / microbiology
  • Dysentery, Bacillary / microbiology
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification*
  • Escherichia coli Infections / microbiology
  • Feces / microbiology
  • Humans
  • Nucleic Acid Amplification Techniques / methods*
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Shigella / genetics
  • Shigella / isolation & purification*
  • Time Factors

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • DNA Probes
  • ipaH protein, Shigella flexneri