Abdominal surgery-induced delayed gastric emptying in rats: role of CRF and sensory neurons

Am J Physiol. 1992 Apr;262(4 Pt 1):G616-20. doi: 10.1152/ajpgi.1992.262.4.G616.


The role of peripheral corticotropin-releasing factor (CRF) and afferent pathways in mediating abdominal surgery-induced delayed gastric emptying was investigated using an intravenous injection of the competitive CRF antagonist alpha-helical CRF9-41 and capsaicin pretreatment in adult fasted rats. Gastric emptying of a non-caloric solution was measured by the phenol red method. Intravenous CRF (0.13 nmol) inhibited by 65% gastric emptying. CRF effect was completely reversed by simultaneous intravenous injection of alpha-helical CRF9-41 (13 nmol). Abdominal surgery under enflurane anesthesia inhibited gastric emptying by 60% measured 3 h postoperatively. Enflurane anesthesia alone had no effect on gastric emptying 3 h after exposure. Abdominal surgery-induced delay of gastric emptying was reversed by 46, 60, and 100% by capsaicin pretreatment (125 mg/kg, -3 wk), intravenous injection of alpha-helical CRF9-41 (13 nmol), and combined pretreatments with capsaicin and CRF antagonist, respectively. Capsaicin and alpha-helical CRF9-41 given alone or in combination did not influence gastric emptying. These results indicate that peripheral CRF receptors along with capsaicin-sensitive afferent neurons contribute to somatovisceral stress (abdominal surgery)-induced delayed gastric emptying.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Abdomen / surgery*
  • Animals
  • Capsaicin / pharmacology
  • Corticotropin-Releasing Hormone / antagonists & inhibitors
  • Corticotropin-Releasing Hormone / pharmacology
  • Corticotropin-Releasing Hormone / physiology*
  • Gastric Emptying* / drug effects
  • Injections, Intravenous
  • Male
  • Neurons, Afferent / physiology*
  • Peptide Fragments / pharmacology
  • Postoperative Period
  • Rats
  • Rats, Inbred Strains
  • Time Factors


  • Peptide Fragments
  • Corticotropin-Releasing Hormone
  • corticotropin releasing hormone (9-41)
  • Capsaicin