Intermittent hypoxia damages cerebellar cortex and deep nuclei

Neurosci Lett. 2005 Feb 28;375(2):123-8. doi: 10.1016/j.neulet.2004.10.091. Epub 2004 Dec 2.

Abstract

Obstructive sleep apnea patients show cerebellar cortex and deep nuclei gray matter loss, a possible consequence of intermittent hypoxia (IH) accompanying the syndrome. We exposed Sprague-Dawley rats (n=24) to room air only or 10.3% O2, balance N2, alternating every 480 s (240 s duty cycle) with room air for 5, 10, 15, 20 or 30 h (7.5 h per day) during light periods. IH-exposed rats showed increased numbers of damaged Purkinje cells (31.1, 50.5, 54.7, 65.2, and 94.4% for 5, 10, 15, 20 and 30 h groups, respectively; p<0.001 for slopes of the total, swollen/autolysed, and shrunken/dark cell counts), as assessed by hematoxylin and eosin staining. Anti-caspase-3 antibody density increased in the fastigial nuclei subsequent to 5-h exposure. Short-term IH exposure elicits dose-dependent cerebellar Purkinje and fastigial neuron damage.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Atmosphere Exposure Chambers
  • Caspase 3
  • Caspases / metabolism
  • Cell Death / physiology
  • Cerebellar Cortex / pathology*
  • Cerebellar Cortex / physiopathology
  • Cerebellar Nuclei / metabolism
  • Cerebellar Nuclei / pathology*
  • Cerebellar Nuclei / physiopathology
  • Disease Models, Animal
  • Hypoxia, Brain / pathology*
  • Hypoxia, Brain / physiopathology
  • Nerve Degeneration / metabolism
  • Nerve Degeneration / pathology*
  • Purkinje Cells / metabolism
  • Purkinje Cells / pathology
  • Rats
  • Rats, Sprague-Dawley
  • Sleep Apnea Syndromes / pathology
  • Sleep Apnea Syndromes / physiopathology
  • Time Factors

Substances

  • Casp3 protein, rat
  • Caspase 3
  • Caspases