Chrysin Suppresses Lipopolysaccharide-Induced cyclooxygenase-2 Expression Through the Inhibition of Nuclear Factor for IL-6 (NF-IL6) DNA-binding Activity

FEBS Lett. 2005 Jan 31;579(3):705-11. doi: 10.1016/j.febslet.2004.12.048.

Abstract

Chrysin is a natural, biologically active compound extracted from many plants, honey and propolis. It possesses potent anti-inflammation, anti-cancer and anti-oxidation properties. The mechanism by which chrysin suppresses COX-2 expression remains poorly understood. In the present report, we investigated the effect of chrysin on the expression of COX-2 in lipopolysaccharide (LPS)-activated Raw 264.7 cells. Chrysin significantly suppressed the LPS-induced COX-2 protein and mRNA expression in a dose-dependent manner. The ability of chrysin to suppress the expression of the COX-2 was investigated using luciferase reporters controlled by various cis-elements in COX-2 promoter region. Mutational analysis and electrophoretic mobility shift assay verified that nuclear factor for IL-6 was identified as responsible for the chrysin-mediated COX-2 downregulation. These results will provide new insights into the anti-inflammatory and anti-carcinogenic properties of chrysin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • CCAAT-Enhancer-Binding Protein-beta / antagonists & inhibitors*
  • CCAAT-Enhancer-Binding Protein-beta / metabolism
  • Cell Line
  • Cyclooxygenase 2
  • DNA / metabolism*
  • DNA Primers
  • Enzyme Activation
  • Flavonoids / physiology*
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Lipopolysaccharides / pharmacology*
  • Mice
  • Mitogen-Activated Protein Kinases / metabolism
  • Phosphorylation
  • Promoter Regions, Genetic
  • Prostaglandin-Endoperoxide Synthases / genetics
  • Prostaglandin-Endoperoxide Synthases / metabolism*
  • Protein Binding
  • Protein-Tyrosine Kinases / metabolism
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • CCAAT-Enhancer-Binding Protein-beta
  • DNA Primers
  • Flavonoids
  • Lipopolysaccharides
  • RNA, Messenger
  • chrysin
  • DNA
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases
  • Protein-Tyrosine Kinases
  • Mitogen-Activated Protein Kinases