An improved protocol for pulsed-field gel electrophoresis typing of Clostridium difficile

J Med Microbiol. 2005 Feb;54(Pt 2):155-157. doi: 10.1099/jmm.0.45808-0.

Abstract

Pulsed-field gel electrophoresis (PFGE) is the 'gold standard' technique for bacterial typing and has proved to be discriminatory and reproducible for typing Clostridium difficile. Nevertheless, a high proportion of strains are non-typable by this technique due to the degradation of the DNA during the process. The introduction of several modifications in the PFGE standard procedure increased typability from 40% (90 isolates) to 100% (220 isolates) while maintaining the high degree of discrimination and reproducibility of the technique.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques*
  • Clostridium difficile / classification*
  • Clostridium difficile / genetics
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field / methods*
  • Reproducibility of Results

Substances

  • DNA, Bacterial