Cloning and characterization of a human angiotensin II type 1 receptor

Biochem Biophys Res Commun. 1992 Mar 31;183(3):989-95. doi: 10.1016/s0006-291x(05)80288-8.


A human liver cDNA library was screened using a rat type 1 angiotensin II receptor cDNA coding sequence as a probe. cDNA clones were isolated which encoded a protein of 359 amino acids that shared 94.4% and 95.3% identify to rat and bovine type 1 angiotensin II receptors, respectively. Ligand binding studies of the cloned receptor expressed in COS cells suggested that it is pharmacologically a type 1 angiotensin II receptor subtype. Electrophysiological studies of the receptor expressed in Xenopus laevis oocytes revealed that it could functionally couple to a second messenger system leading to the mobilization of intracellular stores of calcium. Southern and Northern blot analyses indicated that the cloned receptor is represented as a single copy in the human genome and is expressed in many tissues of different histogenic origin with the exception of brain, where mRNA transcripts were barely detectable.

MeSH terms

  • Amino Acid Sequence
  • Angiotensin II / genetics*
  • Base Sequence
  • Binding, Competitive
  • Blotting, Northern
  • Blotting, Southern
  • Calcium / metabolism
  • Cells, Cultured
  • Cloning, Molecular
  • Humans
  • Liver / chemistry
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Receptors, Angiotensin / classification
  • Receptors, Angiotensin / genetics*
  • Signal Transduction
  • Tissue Distribution


  • RNA, Messenger
  • Receptors, Angiotensin
  • Angiotensin II
  • Calcium

Associated data

  • GENBANK/D10272
  • GENBANK/D10273
  • GENBANK/D10274
  • GENBANK/D10275
  • GENBANK/D10276
  • GENBANK/M87290
  • GENBANK/S88462
  • GENBANK/S88464
  • GENBANK/S88466
  • GENBANK/S88468