Luteolin induced DNA damage leading to human lung squamous carcinoma CH27 cell apoptosis

Eur J Pharmacol. 2005 Jan 31;508(1-3):77-83. doi: 10.1016/j.ejphar.2004.12.032. Epub 2005 Jan 12.

Abstract

Luteolin is an active compound from the Lonicera japonica (Caprifoliaceae). Luteolin (50 microM)-induced human lung carcinoma CH27 cell apoptosis is a typical apoptosis that was accompanied by a significant DNA condensation and apoptotic body formation. Luteolin-induced apoptosis is related to its ability to change the expression of apoptotic markers, such as caspase-3 (caspase-dependent) and apoptosis-inducing factor (caspase-independent) protein expression. The alkaline microgel electrophoresis technique (comet assay), which is the most sensitive, was used for estimation of the luteolin-induced DNA single strand breaks in this study. DNA-damaging effects of luteolin on DNA single strand breaks have been demonstrated in our study. In this study, luteolin induced S-phase cell cycle arrest and increased the mRNA of DNA repair enzymes such as human MutT homologue, 8-oxoguanine-glycosylase and apurinic endonuclease. Our data suggested that luteolin induces CH27 cell apoptosis by caspase-dependent and -independent pathway and the effect of luteolin on apoptosis of CH27 cells is associated with DNA damage and the expression of DNA repair enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects*
  • Apoptosis / genetics
  • Apoptosis Inducing Factor
  • Blotting, Western
  • Carcinoma, Squamous Cell / drug therapy
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology
  • Caspase 3
  • Caspases / metabolism
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • DNA Damage*
  • DNA Glycosylases / genetics
  • DNA Repair Enzymes / genetics
  • DNA, Neoplasm / drug effects
  • DNA, Neoplasm / genetics
  • DNA-(Apurinic or Apyrimidinic Site) Lyase / genetics
  • Dose-Response Relationship, Drug
  • Flavoproteins / metabolism
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / genetics
  • Lung Neoplasms / pathology
  • Luteolin / pharmacology*
  • Membrane Proteins / metabolism
  • Phosphoric Monoester Hydrolases / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors

Substances

  • AIFM1 protein, human
  • Apoptosis Inducing Factor
  • DNA, Neoplasm
  • Flavoproteins
  • Membrane Proteins
  • RNA, Messenger
  • Phosphoric Monoester Hydrolases
  • DNA Glycosylases
  • oxoguanine glycosylase 1, human
  • CASP3 protein, human
  • Caspase 3
  • Caspases
  • 8-oxodGTPase
  • DNA-(Apurinic or Apyrimidinic Site) Lyase
  • DNA Repair Enzymes
  • Luteolin