Immunohistochemical localization of gastrin-releasing peptide receptor in the mouse brain

Brain Res. 2005 Jan 25;1032(1-2):162-70. doi: 10.1016/j.brainres.2004.10.068.


Gastrin-releasing peptide (GRP) is a mammalian bombesin (BN)-like peptide that binds with high affinity to the GRP receptor (GRP-R). Previous behavioral studies using mice and rats showed that the GRP/GRP-R system mediates learning and memory by modulating neurotransmitter release in the local GABAergic network of the amygdala and the nucleus tractus solitarius (NTS). To date, the precise distribution of GRP-R in the brain has not been elucidated. We used a synthetic peptide derived from mouse GRP-R to generate affinity-purified antibodies to GRP-R and used immunohistochemistry to determine the distribution of GRP-R in the mouse brain. The specificity of anti-GRP-R antibody was confirmed in vitro using COS-7 cells transiently expressing GRP-R and in vivo using GRP-R-deficient and wild-type mouse brain sections. GRP-R immunoreactivity was widely distributed in the isocortex, hippocampal formation, piriform cortex, amygdala, hypothalamus, and brain stem. In particular, GRP-R immunoreactivity was observed in the lateral (LA), central, and basolateral amygdaloid (BLA) nuclei and NTS, which are important regions for memory performance. Double-labeling immunohistochemistry demonstrated that subpopulations of GRP-R are present in GABAergic neurons in the amygdala. Consequently, GRP-R immunoreactivity was observed in the GABAergic neurons of the limbic region. These anatomical results provide support for the idea that the GRP/GRP-R system mediates memory performance by modulating neurotransmitter release in the local GABAergic network.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / chemistry
  • Antibodies / metabolism
  • Antibody Specificity
  • Brain / anatomy & histology
  • Brain / metabolism*
  • COS Cells
  • Chlorocebus aethiops
  • Glial Fibrillary Acidic Protein / metabolism
  • Glutamate Decarboxylase / metabolism
  • Immunohistochemistry / methods*
  • Isoenzymes / metabolism
  • Male
  • Mice
  • Mice, Knockout
  • Microtubule-Associated Proteins / metabolism
  • Phosphopyruvate Hydratase / metabolism
  • Receptors, Bombesin / deficiency
  • Receptors, Bombesin / immunology
  • Receptors, Bombesin / metabolism*
  • Transfection / methods


  • Antibodies
  • Glial Fibrillary Acidic Protein
  • Isoenzymes
  • Microtubule-Associated Proteins
  • Mtap2 protein, mouse
  • Receptors, Bombesin
  • Glutamate Decarboxylase
  • glutamate decarboxylase 1
  • Phosphopyruvate Hydratase