A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium

BMC Infect Dis. 2005 Feb 1;5:6. doi: 10.1186/1471-2334-5-6.

Abstract

Background: Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.

Methods: Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.

Results: Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.

Conclusions: Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Base Sequence
  • Belgium / epidemiology
  • Child
  • Child, Preschool
  • Consensus Sequence
  • Coronavirus / genetics*
  • Coronavirus / isolation & purification
  • Coronavirus Infections / epidemiology
  • Coronavirus Infections / virology*
  • DNA Primers / chemistry
  • Female
  • Humans
  • Incidence
  • Infant
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / chemistry
  • Respiratory Tract Infections / epidemiology
  • Respiratory Tract Infections / virology*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Seasons
  • Sequence Alignment

Substances

  • DNA Primers
  • RNA, Viral

Associated data

  • GENBANK/AY758276
  • GENBANK/AY758277
  • GENBANK/AY758278
  • GENBANK/AY758279
  • GENBANK/AY758280
  • GENBANK/AY758281
  • GENBANK/AY758282
  • GENBANK/AY758283
  • GENBANK/AY758284
  • GENBANK/AY758285
  • GENBANK/AY758286
  • GENBANK/AY758287
  • GENBANK/AY758288
  • GENBANK/AY758289
  • GENBANK/AY758290
  • GENBANK/AY758291
  • GENBANK/AY758292
  • GENBANK/AY758293
  • GENBANK/AY758294
  • GENBANK/AY758295
  • GENBANK/AY758296
  • GENBANK/AY758297
  • GENBANK/AY758298
  • GENBANK/AY758299
  • GENBANK/AY758300
  • GENBANK/AY758301