Protein Domain of Unknown Function DUF1023 Is an alpha/beta Hydrolase

Proteins. 2005 Apr 1;59(1):1-6. doi: 10.1002/prot.20388.

Abstract

Pfam family DUF1023 consists entirely of uncharacterized proteins generated by sequencing the genomes of Actinobacteria (Bateman A., et al., Nucleic Acids Res. 2004;32 Database issue:D138-141.) Utilizing sequence similarity detection methods, we infer homology between DUF1023 and alpha/beta hydrolases. DUF1023 proteins conserve the core secondary structures in alpha/beta hydrolase fold, and share similar catalytic machinery as that of alpha/beta hydrolases. We predict DUF1023 spatial structure and deduce that they function as hydrolases utilizing catalytic Ser-His-Asp triad with the serine as a nucleophile.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans / enzymology
  • Caenorhabditis elegans Proteins / metabolism
  • Databases, Protein
  • Hydrolases / chemistry*
  • Hydrolases / metabolism*
  • Lipase / chemistry
  • Lipase / metabolism
  • Models, Molecular
  • Protein Conformation
  • Rhizomucor / enzymology

Substances

  • Caenorhabditis elegans Proteins
  • Hydrolases
  • Lipase