Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound

Biometals. 2004 Dec;17(6):647-54. doi: 10.1007/s10534-004-1230-z.


Schizosaccharomyces pombe has been assumed not to produce siderophores. Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis, and using reversed-phase HPLC and mass spectrometry analysis, we demonstrate that S. pombe excretes and accumulates intracellularly the hydroxamate-type siderophore ferrichrome. Under iron-limiting conditions, the cellular ferrichrome pool was present in the desferri-form, while under iron-richconditions, in the ferri-form. In contrast to S. pombe, hydroxamate-type siderophores could not be detected intwo other yeast species, Saccharomyces cerevisiae and Candida albicans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Biological Assay
  • Biological Transport
  • Chromatography, High Pressure Liquid
  • Ferrichrome / chemistry*
  • Fungal Proteins
  • Hydroxamic Acids / chemistry
  • Iron / metabolism*
  • Mass Spectrometry
  • Models, Chemical
  • Molecular Sequence Data
  • Phylogeny
  • Protein Structure, Tertiary
  • Saccharomyces cerevisiae / metabolism
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces / physiology


  • Fungal Proteins
  • Hydroxamic Acids
  • Ferrichrome
  • Iron