Shifted, the Drosophila ortholog of Wnt inhibitory factor-1, controls the distribution and movement of Hedgehog

Dev Cell. 2005 Feb;8(2):255-66. doi: 10.1016/j.devcel.2005.01.003.

Abstract

We here identify and characterize an extracellular modulator of Hedgehog signaling in Drosophila, Shifted. Shifted is required for high levels of long-range signaling in the developing wing imaginal disc. Surprisingly, shifted encodes the only Drosophila ortholog of the secreted vertebrate protein Wnt Inhibitory Factor-1 (WIF-1), whose known role is to bind to extracellular Wnts and inhibit their activity. However, Shifted does not regulate Hedgehog signaling by affecting Wingless or Wnt signaling. We show instead that Shifted is a secreted protein that acts over a long distance and is required for the normal accumulation of Hh protein and its movement in the wing. Our data further indicate that Shf interacts with Hh and the heparan sulfate proteoglycans. Therefore, we propose that Shf stabilizes the interaction between Hh and the proteoglycans, an unexpected role for a member of the WIF-1 family.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Alleles
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology
  • Cholesterol / metabolism
  • Drosophila / genetics
  • Drosophila / growth & development
  • Drosophila / physiology*
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology*
  • Female
  • Hedgehog Proteins
  • Heparan Sulfate Proteoglycans / metabolism
  • Humans
  • Male
  • Models, Biological
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Repressor Proteins / genetics
  • Repressor Proteins / physiology
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Wings, Animal / growth & development
  • Wings, Animal / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Drosophila Proteins
  • Hedgehog Proteins
  • Heparan Sulfate Proteoglycans
  • Repressor Proteins
  • WIF1 protein, human
  • hh protein, Drosophila
  • Cholesterol