Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor

J Mol Biol. 1992 Apr 20;224(4):1075-85. doi: 10.1016/0022-2836(92)90470-5.

Abstract

The crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four alpha-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel beta-sheet. The tertiary structure of rhGM-CSF has a topology similar to that of porcine growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Computer Graphics
  • Crystallography
  • Granulocyte-Macrophage Colony-Stimulating Factor / ultrastructure*
  • Humans
  • Hylobates
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Conformation
  • Recombinant Proteins
  • Sequence Alignment
  • X-Ray Diffraction

Substances

  • Recombinant Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor