Detection of Aspergillus fumigatus and a mutation that confers reduced susceptibility to itraconazole and posaconazole by real-time PCR and pyrosequencing

J Clin Microbiol. 2005 Feb;43(2):906-8. doi: 10.1128/JCM.43.2.906-908.2005.

Abstract

A real-time PCR and pyrosequencing method was developed to detect Aspergillus fumigatus in whole blood by amplifying the cyp51A gene and sequencing the codon for glycine 54. Mutations in this codon can result in amino acid substitutions that confer reduced susceptibility to itraconazole and posaconazole.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Aspergillus fumigatus / drug effects*
  • Aspergillus fumigatus / genetics
  • Aspergillus fumigatus / isolation & purification
  • Base Sequence
  • Cytochrome P-450 Enzyme System / chemistry
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA, Fungal / blood
  • Drug Resistance, Fungal / genetics*
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Humans
  • Itraconazole / pharmacology*
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Mutation*
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA / methods
  • Triazoles / pharmacology*

Substances

  • Antifungal Agents
  • DNA, Fungal
  • Fungal Proteins
  • Triazoles
  • Itraconazole
  • posaconazole
  • Cytochrome P-450 Enzyme System
  • cytochrome P-450 CYP51A, Aspergillus