Serological diagnosis of human babesiosis by IgG enzyme-linked immunosorbent assay

Curr Microbiol. 2004 Dec;49(6):385-9. doi: 10.1007/s00284-004-4373-9.

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG antibodies to Babesia microti antigen was developed. B. microti antigens were harvested from experimentally infected hamster blood and used as a coating antigen. The sensitivity and specificity of the IgG ELISA relative to immunofluorescent antibody assay (IFA) testing was 95.5% and 94.1%, respectively. According to the receiver operating characteristic curve analysis, the area under the curve was 0.987. No cross-reactivity of serum samples collected from patients infected with Toxoplasma gondii, Borrelia burgdorferi, Anaplasma phagocytophilum, Bartonella quintana, Dengue virus, or West Nile virus was detected. Cross-reactivity was observed with one of 35 sera from patients infected with Bartonella henselae. These results indicate that the established ELISA methods could be utilized as an accurate measure for the clinical diagnosis of human babesiosis.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Antibodies, Protozoan / blood*
  • Babesia microti / immunology*
  • Babesiosis / diagnosis*
  • Cricetinae
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunoglobulin G / blood*
  • Sensitivity and Specificity

Substances

  • Antibodies, Protozoan
  • Immunoglobulin G