Model of RecA-mediated homologous recognition

Phys Rev Lett. 2004 Dec 31;93(26 Pt 1):268102. doi: 10.1103/PhysRevLett.93.268102. Epub 2004 Dec 20.

Abstract

We consider theoretically the homology search between a long double-stranded DNA and a RecA-single-stranded DNA nucleofilament, emphasizing the polymeric nature of the search and the ability of double-stranded DNA to overcome the difference in pitch between itself and the nucleofilament by thermally activated stretching from the canonical B state to the metastable, stretched S state. Our analytical first-passage-time analysis agrees well with experimental data, predicts new dependencies on the intracellular fluid viscosity and ionic strength, and strongly suggests that initial homologous recognition involves a three base-pair seed.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adenosine Triphosphatases
  • Binding Sites
  • Computer Simulation
  • DNA Helicases
  • DNA, Bacterial / chemistry*
  • Diffusion
  • Models, Chemical*
  • Models, Molecular*
  • Motion
  • Protein Binding
  • Rec A Recombinases / chemistry*
  • Sequence Homology

Substances

  • DNA, Bacterial
  • Rec A Recombinases
  • Adenosine Triphosphatases
  • DNA Helicases