The Cadence contrast pulse sequencing (CPS) technology offers improved clinical information and workflow efficiency during contrast agent examinations. The technology harnesses unique non-linear microbubble energy discovered within the same fundamental frequency band as the transmitted pulses of sound and thus offers improved sensitivity and penetration. An auto tracking contrast quantification (ACQ) technology further complements the CPS detection technology with automatic image alignment during quantification with features such as on-line multiple time intensity curves (TIC). The regional image analysis holds promise of alleviating workflow inefficiency associated with manual alignment of image frames.