Effects of antineoplaston AS2-1 against post-operative lung metastasis in orthotopically implanted colon cancer in nude rat

Oncol Rep. 2005 Mar;13(3):389-95.


We have investigated the efficacy and mechanisms of antineoplaston AS2-1 against post-operative lung metastasis following removal of implanted human colon cancer in nude rat. The influence of AS2-1 on in vitro KM12SM human colon carcinoma cell activities (growth, cell cycle, and apoptosis) was evaluated. AS2-1 was administered perorally after removal of the implanted KM12SM cecal cancer in nude rat. AS2-1 inhibited KM12SM cell proliferation through G1 cell arrest and, at a higher concentration, induction of apoptosis. AS2-1 showed significant reduction in lung metastasis at 5 weeks after cecal removal. The survival rate in the AS2-1 group was significantly higher than that in the control. TUNEL staining on the lung metastatic tumors revealed that the apoptosis index (AI) in the AS2-1 group was significantly higher. Antineoplaston AS2-1 showed an antimetastatic effect against post-operative lung metastases from colon cancer through G1 cell arrest and the subsequent induction of apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma / prevention & control*
  • Carcinoma / secondary*
  • Carcinoma / veterinary
  • Colonic Neoplasms / pathology*
  • Colonic Neoplasms / surgery*
  • Colonic Neoplasms / veterinary
  • Drug Combinations
  • G1 Phase / drug effects
  • Glutamine / analogs & derivatives*
  • Glutamine / pharmacology*
  • In Situ Nick-End Labeling
  • Lung Neoplasms / prevention & control*
  • Lung Neoplasms / secondary*
  • Lung Neoplasms / veterinary
  • Male
  • Neoplasms, Experimental
  • Phenylacetates / pharmacology*
  • Rats
  • Rats, Inbred F344


  • Drug Combinations
  • Phenylacetates
  • Glutamine
  • antineoplaston AS 2-1