Objective: To study the phenylethanoid glycosides from root of Picrorhiza scrophulariiflora.
Method: Column chromatographic techniques were used for isolation and purification of chemical constituents of the plant and the structures were identified by spectroscopic analysis.
Result: Six phenylethanoid glycosides were isolated and elucidated as: 2-(3,4-dihydroxyphenyl)-ethyl-O-beta-D-glucopyranoside (1), 2-(3-hydroxy-4-methoxyphenyl)-ethyl-O-beta-D-glucopyranosyl (1-->3) beta-D-glucopyranoside (2), scroside B (3), hemiphroside A (4), plantainoside D (5) and scroside A (6), respectively.
Conclusion: Compounds 1, 2, 4 and 5 were isolated from this plant for the first time and compound 2 was firstly obtained from natural source.