An aqueous Chlorella extract inhibits IL-5 production by mast cells in vitro and reduces ovalbumin-induced eosinophil infiltration in the airway in mice in vivo

Int Immunopharmacol. 2005 Apr;5(4):689-98. doi: 10.1016/j.intimp.2004.11.016.


An aqueous extract of the edible microalga (CP) (1), has recently been tested for its immunomodulatory effects in a human clinical trial. Here, the CP extract was dialyzed and fractionated using Sephadex G 100 chromatography. The effects of a dialyzed aqueous CP extract, fraction 2 , on mast cell mediator release in vitro and ovalbumin-induced allergic airway inflammation in vivo were examined. In vitro, treatment of mouse bone marrow-derived mast cells with 2 for 18 h significantly inhibited antigen (trinitrophenyl-BSA)-induced IL-5 production. In vivo, treatment of mice with 2 during ovalbumin sensitization and stimulation process significantly reduced eosinophil and neutrophil infiltration in the airways. Moreover, fractions obtained by size exclusion chromatography of 2 inhibited IgE-dependent cytokine GM-CSF production from human cord blood-derived mast cells. Taken together, these results suggest that 2 is composed of biopolymers with anti-allergic potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Chlorella / chemistry*
  • Dietary Supplements*
  • Eosinophils / drug effects
  • Eosinophils / physiology
  • Female
  • Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
  • Immunoglobulin E / immunology
  • Interleukin-5 / biosynthesis*
  • Lung / drug effects*
  • Lung / immunology*
  • Mast Cells / drug effects*
  • Mast Cells / physiology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Neutrophils / drug effects
  • Neutrophils / physiology
  • Ovalbumin


  • Interleukin-5
  • Immunoglobulin E
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Ovalbumin