Small Jab1-containing subcomplex is regulated in an anchorage- and cell cycle-dependent manner, which is abrogated by ras transformation

FEBS Lett. 2005 Feb 14;579(5):1047-54. doi: 10.1016/j.febslet.2004.12.076. Epub 2005 Jan 13.


Jab1 interacts with a variety of cell cycle and signal transduction regulators to control cell proliferation, differentiation, and tumorigenesis. In this study, we employed a non-denaturing gel electrophoresis method to separate different Jab1-containing complexes, the COP9 signalosome complex and the small Jab1-containing subcomplex. The formation of the small Jab1 complex was dependent on a low cell density and anchorage to a solid support, and enhanced during the early G1 phase of the cell cycle, which was abrogated in ras-transformed cells. The small Jab1-containing subcomplex may be a novel mediator of anchorage and cell-cell contact-dependent signal transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COP9 Signalosome Complex
  • Cell Adhesion
  • Cell Count
  • Cell Cycle*
  • Cell Line
  • Cell Transformation, Neoplastic*
  • Contact Inhibition
  • DNA-Binding Proteins / metabolism*
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Multiprotein Complexes / chemistry
  • Multiprotein Complexes / isolation & purification
  • Multiprotein Complexes / metabolism
  • Peptide Hydrolases
  • Protein Binding
  • Transcription Factors / metabolism*
  • ras Proteins / genetics
  • ras Proteins / metabolism*


  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • Multiprotein Complexes
  • Transcription Factors
  • Peptide Hydrolases
  • Cops5 protein, mouse
  • COP9 Signalosome Complex
  • ras Proteins