Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase

FEBS Lett. 2005 Feb 14;579(5):1243-8. doi: 10.1016/j.febslet.2005.01.018. Epub 2005 Jan 24.


We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstadienes / pharmacology
  • Arabidopsis / cytology
  • Arabidopsis / drug effects
  • Arabidopsis / enzymology
  • Arabidopsis / metabolism
  • Chromones / pharmacology
  • Enzyme Activation / drug effects
  • Gravitropism
  • Indoleacetic Acids / pharmacology*
  • Morpholines / pharmacology
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Plant Roots / cytology
  • Plant Roots / drug effects
  • Plant Roots / metabolism
  • Protein Kinase Inhibitors / pharmacology
  • Reactive Oxygen Species / metabolism*
  • Wortmannin
  • Zea mays / cytology
  • Zea mays / drug effects
  • Zea mays / enzymology
  • Zea mays / metabolism


  • Androstadienes
  • Chromones
  • Indoleacetic Acids
  • Morpholines
  • Protein Kinase Inhibitors
  • Reactive Oxygen Species
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • Phosphatidylinositol 3-Kinases
  • Wortmannin