Abstract
SJL mice immunized with mannosylated (M-) PLP(139-151) in complete adjuvant do not develop EAE and little CNS mononuclear cell infiltration; other mannosylated peptides were ineffective in this experimental setting. Despite apparently normal T cell responses, M-PLP(139-151)-immunized mice show impaired delayed-type-sensitivity to PLP(139-151) but a normal response to other peptides. After re-immunization with PLP(139-151) in complete adjuvant, these mice are largely tolerant to EAE, show less T cell proliferation and decreased peptide-specific IgG2a. Our data suggest that M-PLP(139-151) induces peptide-specific tolerance to EAE via a mechanism of deletion or impaired migration of encephalitogenic T cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Movement / immunology
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Cells, Cultured
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Central Nervous System / immunology
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Central Nervous System / pathology
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Encephalomyelitis, Autoimmune, Experimental / immunology*
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Encephalomyelitis, Autoimmune, Experimental / pathology
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Encephalomyelitis, Autoimmune, Experimental / prevention & control*
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Female
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Hypersensitivity, Delayed / immunology
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Hypersensitivity, Delayed / pathology
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Immunodominant Epitopes / immunology
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Injections, Subcutaneous
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / pathology
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Mannose / metabolism*
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Mice
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Molecular Sequence Data
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Myelin Proteolipid Protein / administration & dosage*
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Myelin Proteolipid Protein / immunology*
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Myelin Proteolipid Protein / metabolism
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Peptide Fragments / administration & dosage*
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Peptide Fragments / immunology*
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Peptide Fragments / metabolism
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Self Tolerance / immunology*
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Severity of Illness Index
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Th1 Cells / immunology
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Th1 Cells / pathology
Substances
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Immunodominant Epitopes
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Myelin Proteolipid Protein
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Peptide Fragments
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myelin proteolipid protein (139-151)
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Mannose