A fluorescent assay for chloride transport; identification of a synthetic anionophore with improved activity

Beth A McNally; Atanas V Koulov; Bradley D Smith; Jean-Baptiste Joos; Anthony P Davis

doi:10.1039/b414589e

A fluorescent assay for chloride transport; identification of a synthetic anionophore with improved activity

Chem Commun (Camb). 2005 Feb 28:(8):1087-9. doi: 10.1039/b414589e. Epub 2005 Jan 12.

Authors

Beth A McNally¹, Atanas V Koulov, Bradley D Smith, Jean-Baptiste Joos, Anthony P Davis

Affiliation

¹ Department of Chemistry and Biochemistry, University of Notre Dame, IN 46556, USA.

PMID: 15719125
DOI: 10.1039/b414589e

Abstract

A fluorescent assay based on the chloride-sensitive probe, lucigenin, is developed for monitoring chloride transport into vesicles, and used to compare the effectiveness of three steroid-derived transporters.

MeSH terms

Acridines / chemistry*
Chlorides / analysis*
Chlorides / metabolism*
Fluorescence
Ion Transport
Luminescent Agents / chemistry*
Molecular Structure
Spectrometry, Fluorescence / methods

Substances

Acridines
Chlorides
Luminescent Agents
10,10'-dimethyl-9,9'-biacridinium