A novel replication-independent histone H2a gene in mouse

BMC Genet. 2005 Feb 19;6:10. doi: 10.1186/1471-2156-6-10.


Background: An uncharacterized histone H2a-coding transcript (E130307C13) has been cloned from a mouse full-length cDNA library. This transcript is encoded on chromosome 6, approximately 4 kb upstream of a histone H4 gene, Hist4h4. The proteins encoded by this transcript and the human H2afj mRNA isoform-2 have the highest amino acid similarity. In this paper, we characterize it from the expression pattern given by quantitative RT-PCR.

Results: Quantitative RT-PCR indicated that the gene that encodes E130307C13 (E130307C13) is regulated in a replication-independent manner, and therefore it is H2afj. Certainly, H2afj transcript lacks a stem-loop structure at the 3'-UTR but contains a poly (A) signal. In addition, its promoter region has a different structure from those of the replication-dependent histone H2a genes.

Conclusion: The bioinformatics imply that E130307C13 is a replication-independent H2a gene. In addition, quantitative RT-PCR analysis shows that it is replication-independent. Thus, it is H2afj, a novel replication-independent H2a gene in mouse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle
  • Cell Line
  • Cloning, Molecular
  • Gene Expression Regulation
  • Gene Library
  • Histones / genetics*
  • Mice
  • Nucleic Acid Conformation
  • Phylogeny
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction


  • Histones
  • RNA, Messenger