Study on tobacco components involved in the pyrolytic generation of selected smoke constituents

Food Chem Toxicol. 2005 Apr;43(4):559-68. doi: 10.1016/j.fct.2004.12.011.


The aim of this study was to investigate the contribution of various tobacco components to the generation of smoke constituents using a tobacco pyrolysis model. We analyzed the amounts of primary tobacco components (sugars, protein, polyphenols, alkaloids, organic acids, inorganics etc.) in flue-cured and burley tobacco leaves. Each of the components was added to the tobacco leaves at the 0.5-fold and 1.0-fold amount naturally present in the leaves. The treated tobacco samples were pyrolyzed at 800 degrees C in a nitrogen atmosphere with an infrared image furnace, and the selected smoke constituents (benzo[a]pyrene, hydrogen cyanide, carbonyl compounds, aromatic amines, volatile organic compounds and phenolics) were quantitatively analyzed by several methods, including high performance liquid chromatography (HPLC) and gas chromatography/mass spectrometry (GC-MS). The contribution of each tobacco component to the generation of selected smoke constituents was estimated from a regression line determined by the three yields (no addition, 0.5-fold addition, and 1.0-fold addition). The results of this study can provide useful and comprehensive information on the relationship between tobacco components and selected smoke constituents during pyrolysis.

MeSH terms

  • Gas Chromatography-Mass Spectrometry
  • Incineration
  • Models, Theoretical*
  • Organic Chemicals / analysis
  • Plant Leaves / chemistry
  • Smoke*
  • Tobacco / chemistry*


  • Organic Chemicals
  • Smoke