High-density DNA Probe Arrays for Identification of Staphylococci to the Species Level

J Microbiol Methods. 2005 May;61(2):201-8. doi: 10.1016/j.mimet.2004.11.019. Epub 2005 Jan 11.

Abstract

Rapid and accurate identification and speciation of staphylococci clinical isolates is important for predicting medical pathology. We evaluated the ability of a high-density DNA probe array based on 16S rDNA sequences to identify Staphylococcus species. Correct identification was observed for 185 out of the 201 strains (92%). Of the 33 tested species, the array was able to correctly identify 30 of them. The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • DNA Probes / chemistry
  • DNA Probes / genetics*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction / methods
  • RNA, Ribosomal, 16S / chemistry
  • RNA, Ribosomal, 16S / genetics
  • Staphylococcus / classification*
  • Staphylococcus / genetics
  • Staphylococcus / isolation & purification
  • Superoxide Dismutase / chemistry
  • Superoxide Dismutase / genetics

Substances

  • Bacterial Proteins
  • DNA Probes
  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • SodA protein, Bacteria
  • Superoxide Dismutase