Evaluation of left ventricular function in cardiomyopathic mice by tissue Doppler and color M-mode Doppler echocardiography

Echocardiography. 2005 Mar;22(3):245-53. doi: 10.1111/j.0742-2822.2005.04014.x.

Abstract

Tissue Doppler imaging (TDI) and color M-mode Doppler flow propagation velocity (Vp) are used to assess cardiac function in humans, but the feasibility and applicability of these measurements to murine cardiomyopathic models of heart failure remain unclear. Left ventricular (LV) function was measured by TDI and Vp among mice exhibiting severe dilated cardiomyopathy (TOT), pressure-overload hypertrophy (TAC), and normal controls (NTG). Transmitral flow pattern in TACs and TOTs showed a restrictive filling pattern, but early diastolic mitral annulus velocity was comparable among the three studied groups. Propagation velocity in an anesthetized state was comparable in all three groups. However, while Vp increased in all three groups in the conscious state, the increase in NTGs was statistically greater than in TACs and TOTs. Collectively, results indicate that color M-mode Doppler echocardiography can be used to assess LV function in mice. Furthermore, Vp is depressed by anesthesia, a complication that can lead to misinterpretation of LV function in normal hearts.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anesthetics, Inhalation / administration & dosage
  • Animals
  • Blood Flow Velocity / physiology
  • Cardiac Output / physiology
  • Cardiac Output, Low / diagnostic imaging
  • Cardiomyopathies / diagnostic imaging*
  • Cardiomyopathy, Dilated / diagnostic imaging
  • Cardiomyopathy, Hypertrophic / diagnostic imaging
  • Disease Models, Animal
  • Echocardiography, Doppler, Color*
  • Feasibility Studies
  • Heart Ventricles / diagnostic imaging
  • Male
  • Mice
  • Mitral Valve / diagnostic imaging
  • Myocardial Contraction / drug effects
  • Myocardial Contraction / physiology
  • Ventricular Function, Left / drug effects
  • Ventricular Function, Left / physiology*
  • Wakefulness / physiology

Substances

  • Anesthetics, Inhalation