Cutaneous bioassay of salicylic acid as a keratolytic

Int J Pharm. 2005 Mar 23;292(1-2):187-94. doi: 10.1016/j.ijpharm.2004.11.032. Epub 2005 Jan 26.


Keratolytic efficacy of topical preparations containing salicylic acid was studied in humans utilizing adhesive tape stripping and quantifying SC removal by protein analysis. In combination with tape stripping, squamometry was used to evaluate the influence of salicylic acid on skin surface scaliness and desquamation. Furthermore, skin barrier perturbation and skin irritancy was recorded and related to the dermatopharmacological effect of the preparations. In contrast to squamometry, tape stripping combined with protein analysis was sensitive in detecting keratolytic effect of salicylic acid within hours of application. Importantly, whereas the pH of the preparations only minimally influenced efficacy, local dermatotoxicity was significantly increased at acidic pH. This indicates that the quest to increase the amount of free, non-dissociated SA is, in fact, counterproductive as the more acidic preparations resulted in skin irritation and barrier disruption.

Publication types

  • Comparative Study

MeSH terms

  • Administration, Cutaneous*
  • Adult
  • Dermatology / methods
  • Dermatology / trends
  • Drug Evaluation / methods*
  • Erythema / chemically induced
  • Female
  • Humans
  • Hydrogen-Ion Concentration
  • Keratolytic Agents / administration & dosage
  • Keratolytic Agents / chemistry
  • Keratolytic Agents / therapeutic use*
  • Male
  • Menthol / administration & dosage
  • Menthol / adverse effects
  • Salicylic Acid / administration & dosage
  • Salicylic Acid / adverse effects*
  • Skin Irritancy Tests / methods
  • Skin Physiological Phenomena / drug effects
  • Solutions / administration & dosage
  • Solutions / adverse effects
  • Solutions / chemistry
  • Time Factors
  • Water Loss, Insensible / drug effects
  • Water Loss, Insensible / physiology


  • Keratolytic Agents
  • Solutions
  • Menthol
  • Salicylic Acid