Acquisition, consolidation, reconsolidation, and extinction of eyelid conditioning responses require de novo protein synthesis

J Neurosci. 2005 Feb 23;25(8):2070-80. doi: 10.1523/JNEUROSCI.4163-04.2005.

Abstract

Memory, as measured by changes in an animal's behavior some time after learning, is a reflection of many processes. Here, using a trace paradigm, in mice we show that de novo protein synthesis is required for acquisition, consolidation, reconsolidation, and extinction of classically conditioned eyelid responses. Two critical periods of protein synthesis have been found: the first, during training, the blocking of which impaired acquisition; and the second, lasting the first 4 h after training, the blocking of which impaired consolidation. The process of reconsolidation was sensitive to protein synthesis inhibition if anisomycin was injected before or just after the reactivation session. Furthermore, extinction was also dependent on protein synthesis, following the same temporal course as that followed during acquisition and consolidation. This last fact reinforces the idea that extinction is an active learning process rather than a passive event of forgetting. Together, these findings demonstrate that all of the different stages of memory formation involved in the classical conditioning of eyelid responses are dependent on protein synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anisomycin / administration & dosage
  • Anisomycin / pharmacology
  • Association Learning / drug effects
  • Association Learning / physiology*
  • Blinking / drug effects
  • Blinking / physiology*
  • Brain / drug effects*
  • Brain / metabolism
  • Conditioning, Classical / drug effects
  • Conditioning, Classical / physiology*
  • Convulsants / pharmacology
  • DNA-Binding Proteins
  • Drug Administration Schedule
  • Electromyography
  • Electroshock
  • Extinction, Psychological / drug effects
  • Extinction, Psychological / physiology*
  • Eyelids / physiology*
  • Gene Expression Regulation* / drug effects
  • Genes, fos
  • Hippocampus / drug effects
  • Hippocampus / metabolism
  • Kainic Acid / pharmacology
  • Locomotion
  • Memory / drug effects
  • Memory / physiology*
  • Memory, Short-Term / drug effects
  • Memory, Short-Term / physiology
  • Mice
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Nuclear Proteins / analysis
  • Protein Synthesis Inhibitors / administration & dosage
  • Protein Synthesis Inhibitors / pharmacology
  • Proto-Oncogene Proteins c-fos / biosynthesis
  • Time Factors

Substances

  • Convulsants
  • DNA-Binding Proteins
  • Nerve Tissue Proteins
  • NeuN protein, mouse
  • Nuclear Proteins
  • Protein Synthesis Inhibitors
  • Proto-Oncogene Proteins c-fos
  • Anisomycin
  • Kainic Acid