Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Vaccine. 2005 Mar 14;23(16):1986-92. doi: 10.1016/j.vaccine.2004.10.017.


Burkholderia mallei is an obligate mammalian pathogen that causes the zoonotic disease glanders. Two live attenuated B. mallei strains, a capsule mutant and a branched-chain amino acid auxotroph, were evaluated for use as vaccines against aerosol-initiated glanders in mice. Animals were aerogenically vaccinated and serum samples were obtained before aerosol challenge with a high-dose (>300 times the LD50) of B. mallei ATCC 23344. Mice vaccinated with the capsule mutant developed a Th2-like Ig subclass antibody response and none survived beyond 5 days. In comparison, the auxotrophic mutant elicited a Th1-like Ig subclass antibody response and 25% of the animals survived for 1 month postchallenge. After a low-dose (5 times the LD50) aerosol challenge, the survival rates of auxotroph-vaccinated and unvaccinated animals were 50 and 0%, respectively. Thus, live attenuated strains that promote a Th1-like Ig response may serve as promising vaccine candidates against aerosol infection with B. mallei.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aerosols
  • Animals
  • Antibodies, Bacterial / analysis
  • Antibodies, Bacterial / biosynthesis
  • Bacterial Vaccines / administration & dosage*
  • Bacterial Vaccines / immunology*
  • Burkholderia mallei / immunology*
  • DNA, Bacterial / immunology
  • Female
  • Genetic Vectors
  • Glanders / immunology*
  • Glanders / mortality
  • Glanders / prevention & control*
  • Immunity, Cellular / immunology
  • Immunoglobulin G / biosynthesis
  • Mice
  • Mice, Inbred BALB C
  • Plasmids
  • Reverse Transcriptase Polymerase Chain Reaction
  • Survival Analysis
  • Th1 Cells / immunology
  • Th2 Cells / immunology
  • Vaccination / methods*
  • Vaccines, Attenuated / administration & dosage
  • Vaccines, Attenuated / immunology


  • Aerosols
  • Antibodies, Bacterial
  • Bacterial Vaccines
  • DNA, Bacterial
  • Immunoglobulin G
  • Vaccines, Attenuated