Comparison of chemopreventive effects of Vitamin E plus selenium versus melatonin in 7,12-dimethylbenz(a)anthracene-induced mouse brain damage

Cancer Detect Prev. 2005;29(1):54-8. doi: 10.1016/j.cdp.2004.06.006. Epub 2004 Dec 10.

Abstract

In this work, the protective effect of Vitamin E plus selenium (Vit E+Se) and melatonin against 7,12-dimethylbenz(a)anthracene (7,12-DMBA)-induced changes in superoxide dismutase (SOD), glutathione peroxidase (GSHPx), catalase (CAT) and carbonic anhydrase (CA) activities and malonedialdehyde (MDA) levels of mouse brain were compared. 12-month old mice were divided into four groups each including 10 animals. The first group served as control group. The second group was treated with 7,12-DMBA (20 mg/(kg day)). The third group was treated with 7,12-DMBA and Vitamin E (90 microg/(individual day)) and selenium (1.8 microg/(individual day)) simultaneously. The fourth group was treated with 7,12-DMBA and melatonin (4.2 mg/(kg day)) simultaneously. Treatment continued for 21 days after which the mice were sacrificed and brain homogenates were prepared. 7,12-DMBA treated group exhibited significantly decreased levels of brain SOD, GSHPx, CAT and CA activities and increased MDA levels as compared to control. Vitamin E+Se fully or partially restored enzyme inhibition except for SOD. Lipid peroxidation was also reduced in Vitamin E+Se treated group. Melatonin provided a better protection for SOD, GSHPx and CAT, and a plausible protection for CA activity. Protection against lipid peroxidation measured as MDA in melatonin treated group was appreciable although slightly lesser than the protection provided by Vitamin E+Se. The results imply that Vitamin E+Se and melatonin both provide chemoprevention against 7,12-DMBA-induced oxidative stress in mouse brain.

Publication types

  • Comparative Study

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene / administration & dosage
  • 9,10-Dimethyl-1,2-benzanthracene / toxicity*
  • Animals
  • Antioxidants / pharmacology*
  • Brain / enzymology
  • Brain / pathology*
  • Carbonic Anhydrases / pharmacology
  • Carcinogens / administration & dosage
  • Carcinogens / toxicity*
  • Catalase / pharmacology
  • Disease Models, Animal
  • Female
  • Glutathione Peroxidase / pharmacology
  • Malondialdehyde / analysis
  • Melatonin / pharmacology*
  • Mice
  • Oxidative Stress
  • Selenium / pharmacology*
  • Superoxide Dismutase / pharmacology
  • Vitamin E / pharmacology*

Substances

  • Antioxidants
  • Carcinogens
  • Vitamin E
  • Malondialdehyde
  • 9,10-Dimethyl-1,2-benzanthracene
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Carbonic Anhydrases
  • Selenium
  • Melatonin