Separation of aceclofenac and diclofenac in human plasma by free zone capillary electrophoresis using N-methyl-D-glucamine as an effective electrolyte additive

Eur J Pharm Sci. 2005 Mar;24(4):375-80. doi: 10.1016/j.ejps.2004.12.003. Epub 2005 Jan 23.


Aceclofenac (A) and diclofenac (D) are effective non-steroidal anti-inflammatory drugs (NSAIDs) derived from the phenylacetic acid with pronounced antirheumatic, anti-inflammatory, analgesic and antipyretic properties. Our work proposes a new, fast-free zone capillary electrophoresis method for the simultaneous determination of aceclofenac and diclofenac in human plasma. The effect of increasing concentrations of N-methyl-D-glucamine organic base on borate run buffer was investigated. A good separation was achieved using a 40 cm x 75 microm uncoated silica capillary, 300 mmol/l sodium borate buffer, 200 mmol/l N-methyl-D-glucamine, pH 8.9, in about 3 min. Moreover, the plasma sample pre-treatment procedure was examined: acidic precipitants such as trichloroacetic acid (TCA), metaphosphoric acid (MPA), perchloric acid (PCA) or 5-sulphosalicylic acid (SSA) cause a total loss of analytes while acetonitrile allows a recovery of 97-98% of both compounds. Its simplicity and rapidity and the low analysis costs demonstrate that our method is a reliable and efficient mean for the comprehensive determination of aceclofenac and diclofenac in human plasma when pharmacokinetics studies are required.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Diclofenac / analogs & derivatives*
  • Diclofenac / blood*
  • Diclofenac / chemistry
  • Electrolytes / chemistry*
  • Electrophoresis, Capillary / methods
  • Gluconates / chemistry*
  • Humans
  • Male
  • Meglumine / analogs & derivatives*
  • Meglumine / chemistry*


  • Electrolytes
  • Gluconates
  • N-methylglucamine gluconate
  • Diclofenac
  • Meglumine
  • aceclofenac