Development of a monoclonal blocking ELISA for the detection of antibody to Mycoplasma bovis in dairy cattle and comparison to detection by PCR

Vet Immunol Immunopathol. 2005 Apr 8;104(3-4):183-93. doi: 10.1016/j.vetimm.2004.11.008.

Abstract

A monoclonal antibody blocking enzyme-linked immunosorbent assay (B-ELISA) was developed to detect antibodies to Mycoplasma bovis in cattle sera. The assay was highly specific and sensitive and there was no cross-reaction detected. This method revealed a high prevalence of antibodies (60%) to M. bovis in dairy cattle in North Queensland. The diagnostic potential of this B-ELISA for the detection of antibody to M. bovis was compared with its detection by PCR. There was a strong positive correlation between PCR and B-ELISA titers. Thus, the B-ELISA appears to be a valuable and reproducible tool in the serodiagnosis of M. bovis infection in cattle.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood*
  • Antibodies, Monoclonal* / biosynthesis
  • Blotting, Western / veterinary
  • Cattle
  • Cattle Diseases / diagnosis
  • Cattle Diseases / microbiology*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Milk / microbiology
  • Mycoplasma Infections / blood
  • Mycoplasma Infections / microbiology
  • Mycoplasma Infections / veterinary*
  • Mycoplasma bovis / immunology
  • Mycoplasma bovis / isolation & purification*
  • Queensland
  • Rabbits
  • Reproducibility of Results
  • Respiratory Tract Diseases / blood
  • Respiratory Tract Diseases / microbiology
  • Respiratory Tract Diseases / veterinary*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • DNA, Bacterial