Enhanced bone regeneration with a synthetic cell-binding peptide--in vivo results

Biochem Biophys Res Commun. 2005 Apr 8;329(2):789-95. doi: 10.1016/j.bbrc.2005.01.157.


This in vivo study compared the regenerative processes within defined defects of the porcine skull after delivery of a porous algae-derived hydroxyapatite (adHA), a similar, experimental adHA carrying the cell binding peptide P-15, used solely and in combination with 25% autogenous bone (AB). Particulated AB served as a control group. During an observation period of 26 weeks, microradiography and histology were performed at four specific times. Significantly higher mineralization rates (p=0.008) were found 4 weeks after application of the bioactive material in combination with AB. At 12 weeks there was a significantly higher mineralization (p=0.036) following the application of the bioactive form alone. This study showed significantly higher mineralization after use of a P-15 bioactivated material at early stages. Thus, it can be concluded that the application of the P-15 sequence to an hydroxyapatite accelerates the process of early bone formation, whereas no long-term effect was traced.

MeSH terms

  • Animals
  • Bone Regeneration / drug effects*
  • Bone Substitutes / administration & dosage*
  • Bone Substitutes / chemistry*
  • Collagen / administration & dosage*
  • Drug Delivery Systems / methods
  • Fracture Healing / drug effects*
  • Peptide Fragments / administration & dosage*
  • Skull Fractures / pathology*
  • Skull Fractures / therapy*
  • Swine
  • Treatment Outcome


  • Bone Substitutes
  • Peptide Fragments
  • cell-binding peptide P-15
  • Collagen