mCD24 regulates proliferation of neuronal committed precursors in the subventricular zone

Mol Cell Neurosci. 2005 Mar;28(3):462-74. doi: 10.1016/j.mcn.2004.10.007.


We previously showed that deletion of the cell surface molecule mCD24 resulted in an increased proliferation in adult subventricular zone (SVZ). Here, we report an increased PSA-NCAM+/TuJ1- population in the mCD24-/- in vivo SVZ as well as in vitro neurospheres. Isolated in vitro, these cells were able to generate neurospheres. Proliferation studies, using BrdU incorporation, showed an increased proliferation in P7 mCD24-/- SVZ and neurospheres. Using electron microscopy, the same cell types were identified in the in vivo SVZ as well as in vitro neurospheres from the WT and mCD24-/- mice. In mixed neurospheres, formed with WT and EGFP/KO cells (enhanced green fluorescent protein mCD24-/-), the WT environment was able to control the proliferation rate of the mCD24-/- cells, but was unable to regulate their differentiation. We concluded that mCD24 acts cell nonautonomously to regulate transit-amplifying cells proliferation and/or differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Antigens, CD / physiology*
  • CD24 Antigen
  • Cell Differentiation / physiology*
  • Cell Lineage / physiology
  • Cell Proliferation*
  • Cells, Cultured
  • Green Fluorescent Proteins
  • Lateral Ventricles / growth & development*
  • Lateral Ventricles / metabolism*
  • Lateral Ventricles / ultrastructure
  • Male
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Membrane Glycoproteins / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Electron, Transmission
  • Neural Cell Adhesion Molecule L1 / metabolism
  • Neurons / metabolism
  • Neurons / ultrastructure
  • P-Selectin / metabolism
  • Sialic Acids / metabolism
  • Stem Cells / metabolism*
  • Stem Cells / ultrastructure
  • Tubulin / metabolism


  • Antigens, CD
  • CD24 Antigen
  • Cd24a protein, mouse
  • Membrane Glycoproteins
  • Neural Cell Adhesion Molecule L1
  • P-Selectin
  • Sialic Acids
  • Tubulin
  • beta3 tubulin, mouse
  • polysialyl neural cell adhesion molecule
  • Green Fluorescent Proteins