Emerging technologies for gene manipulation in Drosophila melanogaster

Nat Rev Genet. 2005 Mar;6(3):167-78. doi: 10.1038/nrg1553.


The popularity of Drosophila melanogaster as a model for understanding eukaryotic biology over the past 100 years has been accompanied by the development of numerous tools for manipulating the fruitfly genome. Here we review some recent technologies that will allow Drosophila melanogaster to be manipulated more easily than any other multicellular organism. These developments include the ability to create molecularly designed deletions, improved genetic mapping technologies, strategies for creating targeted mutations, new transgenic approaches and the means to clone and modify large fragments of DNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • DNA Transposable Elements
  • Drosophila melanogaster / genetics*
  • Gene Targeting
  • Genes, Insect
  • Genetic Engineering*
  • Mutagenesis*
  • Recombination, Genetic*
  • Transgenes


  • DNA Transposable Elements