The inherent specificity, selectivity, and adaptability of biosensors make them ideal candidates for use throughout the food industry. Potential applications within the supply chain range from testing of foodstuffs for maximum pesticide residue verification through to the routine analysis of analyte(s) concentrations, such as, glucose, sucrose, alcohol, etc., which may be indicators of food quality/acceptability. Biosensor formats include simple "one-shot" disposable devices that can be used either in the field or integrated into more sophisticated laboratory instruments. Until now, the main impact of these devices has been in the medical diagnostics field. However, with ongoing technical development, the food industry will be one of the prime beneficiaries of biosensor technology in the future. This report assesses the current and future trends in the application of biosensors to fresh produce and the wider food industry, focusing on both potential and current target analytes that are fundamental to fresh produce quality, traceability, and safety.