MHC class II interaction with CD4 mediated by a region analogous to the MHC class I binding site for CD8

Nature. 1992 Apr 30;356(6372):796-8. doi: 10.1038/356796a0.


Interactions between major histocompatibility complex (MHC) molecules and the CD4 or CD8 coreceptors have a major role in intrathymic T-cell selection. On mature T cells, each of these two glycoproteins is associated with a class-specific bias in MHC molecule recognition by the T-cell receptor. CD4+ T cells respond to antigen in association with MHC class II molecules and CD8+ T cells respond to antigen in association with MHC class I molecules. Physical interaction between the CD4/MHC class II molecules and CD8/MHC class I molecules has been demonstrated by cell adhesion assay, and a binding site for CD8 on class I has been identified. Here we demonstrate that a region of the MHC class II beta-chain beta 2 domain, structurally analogous to the CD8-binding loop in the MHC class I alpha 3 domain, is critical for function with both mouse and human CD4.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites / physiology
  • CD4 Antigens / immunology*
  • CD8 Antigens / immunology*
  • Computer Simulation
  • Dose-Response Relationship, Immunologic
  • Histocompatibility Antigens Class I / immunology*
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Hybridomas
  • In Vitro Techniques
  • Mice
  • Molecular Sequence Data
  • Oligonucleotides / genetics
  • Transfection


  • CD4 Antigens
  • CD8 Antigens
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Oligonucleotides