Perspective: machines for RNAi

Genes Dev. 2005 Mar 1;19(5):517-29. doi: 10.1101/gad.1284105.


RNA silencing pathways convert the sequence information in long RNA, typically double-stranded RNA, into approximately 21-nt RNA signaling molecules such as small interfering RNAs (siRNAs) and microRNAs (miRNAs). siRNAs and miRNAs provide specificity to protein effector complexes that repress mRNA transcription or translation, or catalyze mRNA destruction. Here, we review our current understanding of how small RNAs are produced, how they are loaded into protein complexes, and how they repress gene expression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Down-Regulation / physiology*
  • Humans
  • Protein Biosynthesis / physiology*
  • RNA Stability / physiology*
  • RNA, Messenger / metabolism*
  • RNA, Small Interfering / metabolism*
  • RNA-Binding Proteins / metabolism*
  • Signal Transduction / physiology
  • Transcription, Genetic / physiology


  • RNA, Messenger
  • RNA, Small Interfering
  • RNA-Binding Proteins