Induction of cell cycle arrest and apoptosis in human non-small cell lung cancer A549 cells by casuarinin from the bark of Terminalia arjuna Linn

Anticancer Drugs. 2005 Apr;16(4):409-15. doi: 10.1097/00001813-200504000-00007.


Casuarinin, a hydrolyzable tannin isolated from the bark of Terminalia arjuna Linn. (Combretaceae), inhibits human non-small cell lung cancer A549 cells by blocking cell cycle progression in the G0/G1 phase and inducing apoptosis. Enzyme-linked immunosorbent assay showed that the G0/G1 phase arrest is due to p53-dependent induction of p21/WAF1. An enhancement in Fas/APO-1 and the two forms of Fas ligand (FasL), membrane-bound FasL and soluble FasL, might be responsible for the apoptotic effect induced by casuarinin. Our study reports here for the first time that the induction of p53 and the activity of the Fas/FasL apoptotic system may participate in the antiproliferative activity of casuarinin in A549 cells.

MeSH terms

  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects*
  • Carcinoma, Non-Small-Cell Lung / drug therapy*
  • Cell Cycle Proteins / metabolism
  • Cell Proliferation
  • Cyclin-Dependent Kinase Inhibitor p21
  • Dose-Response Relationship, Drug
  • Fas Ligand Protein
  • G1 Phase / drug effects*
  • Humans
  • Hydrolyzable Tannins / pharmacology*
  • Lung Neoplasms / drug therapy
  • Membrane Glycoproteins / metabolism
  • Plant Bark / chemistry
  • Plant Extracts / pharmacology
  • Receptors, Tumor Necrosis Factor / metabolism
  • Resting Phase, Cell Cycle / drug effects*
  • Terminalia*
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / metabolism
  • fas Receptor


  • Antineoplastic Agents, Phytogenic
  • CDKN1A protein, human
  • Cell Cycle Proteins
  • Cyclin-Dependent Kinase Inhibitor p21
  • FAS protein, human
  • FASLG protein, human
  • Fas Ligand Protein
  • Hydrolyzable Tannins
  • Membrane Glycoproteins
  • Plant Extracts
  • Receptors, Tumor Necrosis Factor
  • Tumor Suppressor Protein p53
  • casuarinin
  • fas Receptor