Purpose: To investigate the effects of LPS on proliferation and ALPase activity of human dental pulp cells (HPCs).
Methods: HPCs were cultivated from connective tissue explants digested with collagenase type I. The expression of mCD14 was determined, and the effects of LPS on proliferation of HPCs at the concentrations of 0.1μg/ml,1μg/ml and 10μg/ml were observed by FCM. The effects of LPS tested on ALPase activity were examined. X(2) test and one-way ANOVA were used in statistical analysis respectively.
Results: The results showed that LPS receptor mCD14 was not present in HPCs. LPS inhibited proliferation of HPCs (P<0.01) and ALPase activity in HPCs (P<0.05) at the concentrations of 0.1μg/ml,1μg/ml and 10μg/ml.
Conclusions: It is concluded that LPS tested inhibits proliferation of HPCs and ALPase activity of HPCs.