Low immunogenicity of in vitro-expanded human neural cells despite high MHC expression

J Neuroimmunol. 2005 Apr;161(1-2):1-11. doi: 10.1016/j.jneuroim.2004.11.016.


The ability to expand human neural precursor cells in vitro offers new possibilities for future cell therapies. However, concern over immunologically based rejection of in vitro-expanded human neural cells confounds their use as donor cells. Here, we demonstrate that the expression of human leukocyte antigen (HLA) class I and II molecules, but not the co-stimulatory proteins CD40, CD80 and CD86, substantially increase during expansion of neurospheres. Furthermore, peripheral lymphocytes were unresponsive when co-cultured with in vitro-expanded neural cells. Taken together, these results suggest a low immunogenicity of these cultured human neural cells despite HLA incompatibility and high HLA expression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Antigens, CD / metabolism
  • Cell Count / methods
  • Cell Proliferation
  • Cells, Cultured
  • Coculture Techniques / methods
  • Embryo, Mammalian
  • Female
  • Fetus
  • Flow Cytometry / methods
  • Gene Expression Regulation, Developmental / physiology*
  • Humans
  • Immunohistochemistry / methods
  • Lymphocyte Activation / physiology
  • Major Histocompatibility Complex / physiology*
  • Neurons / immunology*
  • Pregnancy
  • Prosencephalon / cytology*
  • Prosencephalon / embryology
  • Spinal Cord / cytology*
  • Spinal Cord / embryology
  • Stem Cells / metabolism
  • Time Factors


  • Antigens, CD