Abstract
The central role of T cells in the induction of tolerance to autoantigens has been well documented. However, the role of antigen-presenting cells (APCs) in this process is not yet fully understood. To better understand the contribution of APCs in tolerance, we studied the interaction of purified APCs and CD4(+) T cells in a model of intravenous (i.v.) tolerance to experimental autoimmune encephalomyelitis (EAE). As expected, we found that T cells were tolerized to the autoantigen after i.v. injection. However, purified APCs obtained from MOG-i.v.-treated mice were paradoxically immuno-stimulatory, as they induced a non-specific Th1-type response both in vitro and in vivo. We conclude that blocking such APC activation would enhance the effectiveness of tolerance induction.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigen-Presenting Cells / immunology*
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Antigen-Presenting Cells / physiology
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / physiology
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Cell Proliferation / drug effects
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Coculture Techniques / methods
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Cytokines / genetics
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Cytokines / metabolism
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Disease Models, Animal
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Dose-Response Relationship, Immunologic
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Encephalomyelitis, Autoimmune, Experimental / chemically induced
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Encephalomyelitis, Autoimmune, Experimental / immunology*
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Encephalomyelitis, Autoimmune, Experimental / pathology
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Female
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Flow Cytometry / methods
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Glycoproteins
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Immune Tolerance / physiology*
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Immunization, Passive
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Injections, Intravenous / methods
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Mice
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Mice, Inbred C57BL
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Myelin-Oligodendrocyte Glycoprotein
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Peptide Fragments
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RNA, Messenger / biosynthesis
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Receptors, Cytokine / genetics
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Receptors, Cytokine / metabolism
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Statistics, Nonparametric
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Time Factors
Substances
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Cytokines
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Glycoproteins
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Myelin-Oligodendrocyte Glycoprotein
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Peptide Fragments
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RNA, Messenger
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Receptors, Cytokine
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myelin oligodendrocyte glycoprotein (35-55)