The interleukin-1-related cytokine IL-1F8 is expressed in glial cells, but fails to induce IL-1beta signalling responses

Cytokine. 2005 Mar 21;29(6):245-50. doi: 10.1016/j.cyto.2004.12.002.


The putative new interleukin (IL)-1 family member IL-1F8 (IL-1eta, IL-1H2) has been shown recently to activate mitogen activated protein kinases (MAPKs), extracellular signal-regulated protein kinase (ERK1/2) and c-Jun N-terminal kinase (JNK), and nuclear factor-kappa B (NFkappa B) via a mechanism that requires IL-1Rrp2 expression in cell lines. The aim of this study was to test the hypothesis that IL-1F8 contributes to brain inflammation and injury, by studying its expression and actions in the different cell types of the mouse brain in culture. Messenger RNA for IL-1F8 was detected in neurons and glia (microglial cells, oligodendrocytes progenitor cells and to a lesser extent astrocytes) by RT-PCR. Bacterial lipopolysaccharide (LPS) had no effect on IL-1F8 mRNA levels in mixed glial cultures. Recombinant mouse IL-1beta induced strong activation of ERK1/2, p38, JNK and NFkappa B, and significant release of IL-6 and PGE2, which was blocked by IL-1ra. In contrast, recombinant mouse IL-1F8 did not influence any of these parameters. These results demonstrate that CNS cells may be a source of IL-1F8, but the failure of LPS to modulate IL-1F8 mRNA expression, and of recombinant IL-1F8 to induce any of the classical IL-1 responses, suggest that this cytokine has restricted activities in the brain, or that it may act via alternative pathway(s).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / metabolism
  • Cells, Cultured
  • Dinoprostone / biosynthesis
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Gene Expression
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / genetics
  • Interleukin-1 / pharmacology*
  • Interleukin-6 / biosynthesis
  • Mice
  • NF-kappa B / metabolism
  • Neuroglia / drug effects
  • Neuroglia / immunology*
  • RNA, Messenger / metabolism
  • Recombinant Proteins / pharmacology
  • Signal Transduction


  • IL-1F8 protein, mouse
  • Interleukin-1
  • Interleukin-6
  • NF-kappa B
  • RNA, Messenger
  • Recombinant Proteins
  • Extracellular Signal-Regulated MAP Kinases
  • Dinoprostone