Use of microsatellite markers and gene dosage to quantify gene copy numbers in Candida albicans

J Clin Microbiol. 2005 Mar;43(3):1387-9. doi: 10.1128/JCM.43.3.1387-1389.2005.

Abstract

With microsatellite marker typing, the number of alleles must be known for calculation of allelic frequencies in the diploid Candida albicans for a given locus. We describe a gene dosage with a double real-time PCR. Such a dosage should also be useful in exploring the loss of heterozygosity in C. albicans.

MeSH terms

  • Candida albicans / genetics*
  • Gene Dosage*
  • Microsatellite Repeats*
  • Polymerase Chain Reaction